Comparison of Three Serologic Tests for the Detection of Anti-Coxiella burnetii Antibodies in Patients with Q Fever

The performance of a commercial immunofluorescence assay (IFA commercial), an in-house in-house) and indirect enzyme-linked immunosorbent (ELISA) were evaluated in the detection antibodies anti-C. burnetii serum Q fever patients persons without disease. For study, seropositive seronegative samples for (n = 200) from bank Instituto Adolfo Lutz Brazil used. Commercial IFA was considered this study as gold standard diagnosing fever. demonstrated good agreement with test, showing high sensitivity (91%) specificity (97%) compared to standard, Kappa coefficient 0.8954. ELISA test showed lower low (67%), although technique moderate (0.6631). In-house is excellent alternative